ERBB2 Antibody (OAAF01324)

Catalog No: OAAF01324 (Formerly GWB-ASC709)

Size:100 ug

Western blot analysis of extracts from MDA-MB-231 cells, using HER2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HER2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human HER2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: HER2 (Ab-877) Antibody detects endogenous levels of total HER2 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000
  IHC: 1:50~100
  ELISA: 1:20000
• Reference: Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
  Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
  Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
  Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299.
  Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

Target Info

• Gene Symbol: ERBB2
• Gene Full Name: erb-b2 receptor tyrosine kinase 2
• Alias Symbols: CD340;c-erb B2/neu protein;HER2;HER-2;HER-2/neu;herstatin;human epidermal growth factor receptor 2;metastatic lymph node gene 19 protein;MLN 19;NEU;neuro/glioblastoma derived oncogene homolog;neuroblastoma/glioblastoma derived oncogene homolog;NGL;p185erbB2;proto-oncogene c-ErbB-2;proto-oncogene Neu;receptor tyrosine-protein kinase erbB-2;TKR1;tyrosine kinase-type cell surface receptor HER2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2;v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;VSCN2.
• NCBI Gene Id: 2064
• Protein Name: Receptor tyrosine-protein kinase erbB-2
• Description of Target: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Uniprot ID: P04626
• Protein Accession #: NP_001005862.1
• Molecular Weight: 137 kDa