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Catalog No: OKCD02525
Size:96WELLS
Price: $700.00
SKU
OKCD02525
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Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-105%92-101%81-93%80-96%
EDTA Plasma (n=5)83-104%83-101%79-103%84-95%
Heparin Plasma (n=5)80-89%78-93%88-95%80-101%
ELISA Kit PrincipleThe microtiter plate provided in this kit has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody specific to EPX. After TMB substrate solution is added, those wells that contain EPX will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)98-105102
EDTA Plasma (n=5)89-10193
Heparin Plasma(n=5)90-9895
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Peroxidase (EPX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EPX Microplate96 Wells (12 x 8 Well strips)
EPX Lyophilized Standard2
100X Biotinylated EPX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.0 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eosinophil Peroxidase (EPX). No significant cross-reactivity or interference between Eosinophil Peroxidase (EPX) and analogues was observed
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolEpx
Gene Full Nameeosinophil peroxidase
Alias SymbolsE;eosinophil peroxidase;EPO.
NCBI Gene Id13861
Protein NameEosinophil peroxidase
Description of TargetMediates tyrosine nitration of secondary granule proteins in mature resting eosinophils.
Uniprot IDP49290
Protein Accession #NP_031972.2
Nucleotide Accession #NM_007946.2
Protein Size (# AA)716
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