Epinephrine ELISA Kit (OKEH02561)

Catalog No: OKEH02561

Size:96T

Epinephrine ELISA Kit (OKEH02561) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Epinephrine ELISA Kit (OKEH02561) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Epinephrine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Epinephrine and incubated. The Epinephrine found in the sample or standards competes with the biotinylated Epinephrine for limited binding sites on the immobilized anti-Epinephrine antibody. Excess unbound biotinylated Epinephrine and sample or standard Epinephrine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Epinephrine captured in the well and inversely proportional to the amount of Epinephrine which was contained in the sample or standard.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 97%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • Anti-Epinephrine Microplate: 96 Wells (12 x 8 Well strips)
  • Epinephrine Lyophilized Standard: 2 x 2,000 pg
  • Sample Diluent: 1 x 20 mL
  • 100X Epinephrine-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: EPI
  Target Name: Epinephrine
  Pubchem: 5816
  Chemical formula: C9H13NO3
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 9.9 pg/mL
• Specificity: Natural and recombinant General Epinephrine
• Assay Info: Assay Methodology: Quantitative Competitive ELISA
• Description:
  View product citations for Epinephrine ELISA Kit (OKEH02561) on CiteAb

Target Info

• Alias Symbols: EPI, Epinephrine, EPI, Adrenaline
• Protein Name: Epinephrine
• Description of Target: Epinephrine (also known as adrenaline) is a hormone and a neurotransmitter. Epinephrine has many functions in the body, regulating heart rate, blood vessel and air passage diameters, and metabolic shifts; epinephrine release is a crucial component of the fight-or-flight response of the sympathetic nervous system. In chemical terms, epinephrine is one of a group of monoamines called the catecholamines. It is produced in some neurons of the central nervous system, and in the chromaffin cells of the adrenal medulla from the amino acids phenylalanine and tyrosine.