EPHX2 ELISA Kit (Human) (OKCA01229)

Catalog No: OKCA01229

Size:96 Wells

Human EPHX2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	84-93
  1:2	97	92-101
  1:4	91	85-95
  1:8	93	89-97

• ELISA Kit Principle: Aviva Systems Biology EPHX2 ELISA Kit (Human) (OKCA01229) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPHX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EPHX2 captured in the well.
• ELISA Kit Range: 23.44 - 1,500 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	103	99-108
  EDTA Plasma (n=4)	93	89-97

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01229 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01229 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • EPHX2 Microplate: 96 Wells (12 x 8 Well strips)
  • EPHX2 Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated EPHX2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 5.86 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: EPHX2
• Gene Full Name: epoxide hydrolase 2
• Alias Symbols: CEH, SEH, ABHD20
• NCBI Gene Id: 2053
• Protein Name: Bifunctional epoxide hydrolase 2
• Description of Target: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phosphate.
• Uniprot ID: P34913
• Protein Accession #: NP_001243411.1
• Nucleotide Accession #: NM_001256482.1