Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)

Catalog No: OAAF00520 (Formerly GWB-ASB962)

Size:100 ug

View product citations for Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520) on CiteAb

Western blot analysis of extracts from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Target Modification: Phospho
  Modification Sites: H:Y324/311/318 M:Y323/314/318 R:Y323/-/318
  
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  ELISA: 1:20000
• Reference: Beckmann M.P., EMBO J. 13:3757-3762(1994).
  Davis S., Science 266:816-819(1994).
  Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
• Publications:

  Activating Mutations in PIK3CA Lead to Widespread Modulation of the Tyrosine Phosphoproteome. J. Proteome Res. 14, 3882-91 (2015). 26267517

• Description:
  View product citations for Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520) on CiteAb

Target Info

• Gene Symbol: EFNB1|EFNB2|EFNB3
• Gene Full Name: ephrin B1|ephrin B2|ephrin B3
• Alias Symbols: CFND;CFNS;EFB1;EFL3;EFL-3;EFL6;ELK ligand;Elk-L;EPH-related receptor transmembrane ligand ELK-L3;eph-related receptor tyrosine kinase ligand 2;eph-related receptor tyrosine kinase ligand 5;eph-related receptor tyrosine kinase ligand 8;ephrin-B1;ephrin-B2;ephrin-B3;EPLG2;EPLG5;EPLG8;HTK ligand;Htk-L;HTKL;LERK2;LERK5;LERK8;ligand of eph-related kinase 5.
• NCBI Gene Id: 1947|1948|1949
• Protein Name: Ephrin-B1|Ephrin-B2|Ephrin-B3
• Description of Target: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.|Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
• Uniprot ID: P52799|P98172|Q15768
• Protein Accession #: NP_001397.1
• Molecular Weight: 38 kDa