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Catalog No: OKEH04453
Size:96T
Price: $525.00
SKU
OKEH04453
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology EPHA3 ELISA Kit (Human) (OKEH04453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for EPHA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPHA3 captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-EPHA3 Microplate96 Wells (12 x 8 Well Strips)
EPHA3 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
EPHA3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human Ephrin type-A receptor 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEPHA3
Gene Full NameEPH receptor A3
Alias SymbolsEK4;EPH-like kinase 4;eph-like tyrosine kinase 1;ephrin type-A receptor 3;ETK;ETK1;HEK;HEK4;human embryo kinase 1;testicular tissue protein Li 64;TYRO4;TYRO4 protein tyrosine kinase;tyrosine-protein kinase receptor ETK1;Tyrosine-protein kinase TYRO4.
NCBI Gene Id2042
Protein NameP29320
Description of TargetEphrin type-A receptor 3
Uniprot IDhttps://www.uniprot.org/uniprot/P29320
Protein Accession #NP_005224.2
Nucleotide Accession #NM_005233.5
Protein Size (# AA)983
Molecular Weight110 KDa
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