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Catalog No: OKEH04020
Size:96T
Price: $500.00
SKU
OKEH04020
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ENPP2 ELISA Kit (Human) (OKEH04020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENPP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ENPP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ENPP2 captured in the well.
ELISA Kit Range1.25-80ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.3% (n = 20)
Mean Inter-assay CV%: < 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ENPP2 Microplate96 Wells (12 x 8 Well Strips)
ENPP2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
ENPP2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.98 ng/mL
SpecificityNatural and recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolENPP2
Gene Full Nameectonucleotide pyrophosphatase/phosphodiesterase 2
Alias SymbolsATX;ATX-X;AUTOTAXIN;autotaxin-t;ectonucleotide pyrophosphatase/phosphodiesterase family member 2;E-NPP 2;extracellular lysophospholipase D;LysoPLD;NPP2;PD-IALPHA;PDNP2;phosphodiesterase I/nucleotide pyrophosphatase 2;plasma lysophospholipase D.
NCBI Gene Id5168
Protein NameQ13822
Description of TargetEctonucleotide pyrophosphatase/phosphodiesterase family member 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q13822
Protein Accession #NP_001035181.1
Nucleotide Accession #NM_001040092.2
Protein Size (# AA)863
Molecular Weight99 kDa
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