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Catalog No: OKCD02574
Size:96WELLS
Price: $625.00
SKU
OKCD02574
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Datasheets/ManualsPrintable datasheet for ENPEP ELISA Kit (Mouse) (OKCD02574)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-101%88-95%87-94%84-105%
EDTA Plasma (n=5)86-93%79-96%79-94%94-102%
Heparin Plasma (n=5)91-98%87-94%80-93%92-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminopeptidase A (ENPEP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminopeptidase A (ENPEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminopeptidase A (ENPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aminopeptidase A (ENPEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)97-104101
EDTA Plasma (n=5)91-9894
Heparin Plasma(n=5)86-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminopeptidase A (ENPEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminopeptidase A (ENPEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ENPEP Microplate96 Wells (12 x 8 Well strips)
ENPEP Lyophilized Standard2
100X Biotinylated ENPEP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutamyl Aminopeptidase (GluAP). No significant cross-reactivity or interference between Glutamyl Aminopeptidase (GluAP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolEnpep
Gene Full Nameglutamyl aminopeptidase
Alias Symbols6030431M22Rik;aminopeptidase A;APA;AP-A;Bp-1/6C;Bp-1/6C3;BP-1/6C3 antigen;EAP;glutamyl aminopeptidase;Ly5;Ly-5;Ly51;Ly-51.
NCBI Gene Id13809
Protein NameGlutamyl aminopeptidase
Description of TargetRegulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Uniprot IDP16406
Protein Accession #NP_031960.1
Nucleotide Accession #NM_007934.3
Protein Size (# AA)945
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