ENPEP Antibody (OAEB02454)

Catalog No: OAEB02454 (Formerly GWB-843E09)

Size:100UG

OAEB02454 staining (1ug/ml) of human testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02454 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ENPEP
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 92%; Guinea Pig: 83%; Human: 100%; Mouse: 100%
• Peptide Sequence: C-EQYQKTSLAQEKEK
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 170kDa band observed in human brain and testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3ug/ml.
  Immunohistochemistry: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration, 3-5ug/ml.
• Reference: * Velez JC, Ryan KJ, Harbeson CE, Bland AM, Budisavljevic MN, Arthur JM, Fitzgibbon WR, Raymond JR, Janech MG.; Angiotensin I is largely converted to angiotensin (1-7) and angiotensin (2-10) by isolated rat glomeruli.; Hypertension. 2009 May;53(5):790-7.; Pubmed 19289651
  * Velez JC, Bland AM, Arthur JM, Raymond JR, Janech MG.; Characterization of renin-angiotensin system enzyme activities in cultured mouse podocytes.; Am J Physiol Renal Physiol. 2007 Jul;293(1):F398-407.; Pubmed 17429035
  * Malerba G, Pignatti PF.; A review of asthma genetics: gene expression studies and recent candidates.; J Appl Genet. 2005;46(1):93-104. Review.; Pubmed 15741670

Target Info

• Gene Symbol: Enpep|ENPEP
• Gene Full Name: glutamyl aminopeptidase
• Alias Symbols: 6030431M22Rik;aminopeptidase A;APA;AP-A;Bp-1/6C;Bp-1/6C3;BP-1/6C3 antigen;CD249;differentiation antigen gp160;EAP;glutamyl aminopeptidase;gp160;Ly5;Ly-5;Ly51;Ly-51.
• NCBI Gene Id: 13809|2028
• Protein Name: Glutamyl aminopeptidase
• Uniprot ID: Q07075
• Protein Accession #: NP_001968.3
• Molecular Weight: 109