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Catalog No: OKCA00125
Size:96 Wells
Price: $645.00
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OKCA00125
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ENO2 ELISA Kit (Human) : 96 Wells (OKCA00125)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Human Serum (n=4)1:188%84-92%
Human Serum (n=4)1:294%90-98%
Human Serum (n=4)1:499%95-104%
Human Serum (n=4)1:8102%96-108%
ELISA Kit PrincipleAviva Systems Biology ENO2 ELISA Kit (Human) (OKCA00125) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human ENO2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human ENO2 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human ENO2 captured in well.
ELISA Kit Range0.78 ng/mL - 50 ng/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)92%88-96%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)96%90-102%
ELISA Kit ReproducibilityIntra-assay: CV% < 8% (n = 20).Inter-assay: CV% < 10% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ENO2 Microplate96 Well (12 x 8 Well Strips)
ENO2 Standard (Lyophilized)2 x 50 ng
100X Biotinylated Anti-ENO2Detector Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity< 0.195 ng/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of human
NSE. No significant cross-reactivity or interference between human NSE and
analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human NSE and all the analogues,
therefore, cross reaction may still exist.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolENO2
Gene Full NameEnolase 2 (gamma, neuronal)
Alias SymbolsNSE, HEL-S-279
NCBI Gene Id2026
Protein NameGamma-enolase
Description of TargetThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot IDP09104, Q96J33, A8K3B0
Protein Accession #NP_001966
Nucleotide Accession #NM_001975
Protein Size (# AA)434
Molecular Weight47 kDa
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