ENO1 Antibody - C-terminal region (ARP38031_T100)

Catalog No: ARP38031_T100

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELR
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-ENO1 (ARP38031_T100) antibody is Catalog # AAP38031 (Previous Catalog # AAPP10016)
• Sample Type Confirmation:

  ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330

Target Info

• Gene Symbol: ENO1
• Gene Full Name: Enolase 1, (alpha)
• Alias Symbols: NNE, PPH, MPB1, ENO1L1, HEL-S-17
• NCBI Gene Id: 2023
• Protein Name: Alpha-enolase
• Description of Target: ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
• Uniprot ID: Q53FT9
• Protein Accession #: NP_001419
• Nucleotide Accession #: NM_001428
• Protein Size (# AA): 434
• Molecular Weight: 47 kDa
• Protein Interactions: UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1