Catalog No: OKEH01727
Size:96T
Price: $600.00
SKU
OKEH01727
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ENHO ELISA Kit (Human) (OKEH01727) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ENHO antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ENHO and incubated. The ENHO found in the sample or standards competes with the biotinylated ENHO for limited binding sites on the immobilized anti-human ENHO antibody. Excess unbound biotinylated ENHO and sample or standard ENHO is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ENHO captured in the well and inversely proportional to the amount of ENHO which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ENHO Microplate96 Wells (12 x 8 Well strips)
ENHO Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ENHO-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity31 pg/mL
SpecificityNatural and recombinant Human Adropin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolENHO
Gene Full Nameenergy homeostasis associated
Alias Symbolsadropin;C9orf165;energy homeostasis-associated protein;GAAI470;UNQ470.
NCBI Gene Id375704
Protein NameQ6UWT2
Description of TargetAdropin
Uniprot IDhttps://www.uniprot.org/uniprot/Q6UWT2
Protein Accession #NP_940975.2
Nucleotide Accession #NM_198573.2
Protein Size (# AA)76
Molecular Weight7 kDa
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