EMR1 ELISA Kit (Human) (OKCD02523)

Catalog No: OKCD02523

Size:96WELLS

Human EMR1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-102%	92-101%	90-104%	82-97%
  EDTA Plasma (n=5)	82-101%	88-95%	83-99%	86-101%
  Heparin Plasma (n=5)	97-105%	87-96%	83-95%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.50-4000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-97	92
  EDTA Plasma (n=5)	90-104	97
  Heparin Plasma(n=5)	93-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EMR1 Microplate: 96 Wells (12 x 8 Well strips)
  • EMR1 Lyophilized Standard: 2
  • 100X Biotinylated EMR1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 25.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). No significant cross-reactivity or interference between EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADGRE1
• Gene Full Name: adhesion G protein-coupled receptor E1
• Alias Symbols: adhesion G protein-coupled receptor E1;egf-like module containing, mucin-like, hormone receptor-like 1;egf-like module containing, mucin-like, hormone receptor-like sequence 1;EGF-like module receptor 1;EGF-like module-containing mucin-like hormone receptor-like 1;EMR1;EMR1 hormone receptor;TM7LN3.
• NCBI Gene Id: 2015
• Protein Name: Adhesion G protein-coupled receptor E1
• Description of Target: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
• Uniprot ID: Q14246
• Protein Accession #: NP_001243181.1
• Nucleotide Accession #: NM_001256252.1
• Protein Size (# AA): 886