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Catalog No: OKCD00659 (Formerly GWB-KBBKA9)
Size:96WELLS
Price: $650.00
SKU
OKCD00659
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Datasheets/ManualsPrintable datasheet for EMILIN1 ELISA Kit (Human) (OKCD00659)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)92-99%94-102%90-98%80-93%
EDTA plasma(n=5)89-101%91-99%78-92%80-96%
heparin plasma(n=5)93-105%97-104%85-98%85-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 1 (EMILIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Elastin Microfibril Interface Located Protein 1 (EMILIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-10499
EDTA plasma(n=5)79-10497
heparin plasma(n=5)89-10397
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EMILIN1 Microplate96 Wells (12 x 8 Well Strips)
EMILIN1 Lyophilized Standard2 x 100 ng
100X Biotinylated EMILIN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.56 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 1 (EMILIN1). No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 1 (EMILIN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEMILIN1
Gene Full Nameelastin microfibril interfacer 1
Alias Symbolselastin microfibril interface-located protein 1;EMI;EMILIN;EMILIN-1;gp115.
NCBI Gene Id11117
Protein NameEMILIN-1
Description of TargetMay be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
Uniprot IDQ9Y6C2
Protein Accession #NP_008977
Nucleotide Accession #NM_007046.3
Protein Size (# AA)1016
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