ELL3 Antibody - C-terminal region (OAAB13787)

Catalog No: OAAB13787

Size:400ul

ELL3 Antibody (C-term) (Cat. # OAAB13787) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ELL3 antibody detected the ELL3 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
  Function: Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT) (By similarity). Elongation factors can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA
  Cellular Location: Nucleus.
  Tissue Location: Testis specific.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ELL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-360 amino acids from the C-terminal region of human ELL3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Warning: ELL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: ELL3
• Alias Symbols: ELL3, RNA polymerase II elongation factor ELL3
• NCBI Gene Id: 80237
• Protein Name: RNA polymerase II elongation factor ELL3
• Uniprot ID: Q9HB65
• Protein Accession #: NP_079441.1
• Molecular Weight: 45361 Da