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Datasheets/Manuals | Printable datasheet for EIF5B Antibody (OAAF03661) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human EIF5B. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | EIF5B antibody detects endogenous levels of total EIF5B protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:40000 |
Reference | Nagase T., DNA Res. 5:277-286(1998). Wilson S.A., Biochem. J. 342:97-103(1999). Lee J.H., Proc. Natl. Acad. Sci. U.S.A. 96:4342-4347(1999). |
Gene Symbol | EIF5B |
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Gene Full Name | eukaryotic translation initiation factor 5B |
Alias Symbols | eIF-5B;eukaryotic translation initiation factor 5B;IF2;translation initiation factor IF2;translation initiation factor IF-2. |
NCBI Gene Id | 9669 |
Protein Name | Eukaryotic translation initiation factor 5B |
Description of Target | Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes. |
Uniprot ID | O60841 |
Protein Accession # | NP_056988.3 |
Molecular Weight | 138 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF5B Antibody (OAAF03661)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "EIF5B Antibody (OAAF03661)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EIF5B Antibody (OAAF03661)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF5B Antibody (OAAF03661)"?
This target may also be called "eIF-5B;eukaryotic translation initiation factor 5B;IF2;translation initiation factor IF2;translation initiation factor IF-2." in publications.
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What is the shipping cost for "EIF5B Antibody (OAAF03661)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF5B Antibody (OAAF03661)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF5B Antibody (OAAF03661)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "138 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF5B Antibody (OAAF03661)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF5B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF5B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF5B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF5B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF5B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF5B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.