EIF3E Antibody (OAEB00992)

Catalog No: OAEB00992 (Formerly GWB-EV1044)

Size:100UG

OAEB00992 (0.03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Porcine
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: C Terminus
  Immunogen: Peptide with sequence C-KLNQNSRSEAPN, from the C Terminus of the protein sequence according to NP_001559.1.
  Reactivity Info: Tested: Human; Expected: Human, Dog, Cow, Pig
• Reconstitution and Storage: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: EIF3E
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-KLNQNSRSEAPN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:2000.
  Western Blot: Approx 50kDa band observed in lysates of cell line Daudi (calculated MW of 52.2kDa according to NP_001559.1). Recommended concentration: 0.03-0.1ug/ml.
• Reference: Zhou M, Sandercock AM, Fraser CS, Ridlova G, Stephens E, Schenauer MR, Yokoi-Fong T, Barsky D, Leary JA, Hershey JW, Doudna JA, Robinson CV, ; Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3.; Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 105 (47): 18139-44.; PMID: 18599441

Target Info

• Gene Symbol: EIF3E
• Gene Full Name: eukaryotic translation initiation factor 3 subunit E
• Alias Symbols: eIF-3 p48;eIF3-p46;EIF3-P48;EIF3S6;eukaryotic translation initiation factor 3 subunit 6;eukaryotic translation initiation factor 3 subunit E;eukaryotic translation initiation factor 3, subunit 6 (48kD);eukaryotic translation initiation factor 3, subunit 6 48kDa;INT6;mammary tumor-associated protein INT6;murine mammary tumor integration site 6 (oncogene homolog);viral integration site protein INT-6 homolog.
• NCBI Gene Id: 3646
• Protein Name: Eukaryotic translation initiation factor 3 subunit E
• Uniprot ID: P60228
• Protein Accession #: NP_001559.1
• Molecular Weight: 52.2