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Catalog No: OKEH03983
Size:96T
Price: $675.00
SKU
OKEH03983
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology EIF2C2 ELISA Kit (Bovine) (OKEH03983) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EIF2C2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EIF2C2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EIF2C2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Component
ComponentAmount
EIF2C2 Microplate96 Wells (12 x 8 Well strips)
EIF2C2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated EIF2C2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity6 pg/mL
SpecificityNatural and recombinant Bovine Protein argonaute-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAGO2
Gene Full Nameargonaute RISC catalytic component 2
Alias Symbolsargonaute 2, RISC catalytic component;Argonaute RISC catalytic component 2;EIF2C2;Eukaryotic translation initiation factor 2C 2;eukaryotic translation initiation factor 2C, 2;protein argonaute-2;Protein slicer.
NCBI Gene Id404130
Protein NameQ6QME8
Description of TargetProtein argonaute-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q6QME8
Protein Accession #NP_991363.1
Nucleotide Accession #NM_205794.1
Protein Size (# AA)860
Molecular Weight860 kDa
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