EIF2B4 Antibody (OAEB01394)

Catalog No: OAEB01394 (Formerly GWB-EV0932)

Size:100UG

OAEB01394 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: EIF2B4
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 84%
• Peptide Sequence: C-RKDYGSKVSLFSH
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 60kDa band observed in lysates of cell line K562 (calculated MW of 59.6kDa according to NP_751945.2). Recommended concentration: 0.3-1ug/ml.
  An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Wu Y, Pan Y, Du L, Wang J, Gu Q, Gao Z, Li J, Leng X, Qin J, Wu X, Jiang Y,; Identification of novel EIF2B mutations in Chinese patients with vanishing white matter disease.; Journal of human genetics 2009 Feb 54 (2): 74-7.; Pubmed 19158808

Target Info

• Gene Symbol: Eif2b4|EIF2B4
• Gene Full Name: eukaryotic translation initiation factor 2B subunit delta|eukaryotic translation initiation factor 2B, subunit 4 delta
• Alias Symbols: Eif2;Eif2b;EIF-2B;eIF-2B GDP-GTP exchange factor subunit delta;EIF2Bdelta;eukaryotic translation initiation factor 2B subunit 4;eukaryotic translation initiation factor 2B subunit 4 delta;eukaryotic translation initiation factor 2B, subunit 4 delta;eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa;translation initiation factor eIF-2b delta subunit;translation initiation factor eIF-2B subunit delta.
• NCBI Gene Id: 117019|13667|8890
• Protein Name: Eukaryotic translation initiation factor 2B, subunit 4 delta long isoform variant EMBL BAD92769.1
• Uniprot ID: Q59FC8
• Protein Accession #: NP_751945.2
• Molecular Weight: 59.6; 57.6; 57.5