EIF2B2 Antibody (OAGA01031)

Catalog No: OAGA01031

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
OAGA01031 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Colon ca, using EIF2B beta(OAGA01031) antibody at 1:250 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM]
  Positive Controls: 293T, A431, HeLa, HepG2, Molt-4, Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 88 and 323 of EIF2B beta (Uniprot ID#P49770)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.43 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: EIF2B2
• Gene Full Name: Translation initiation factor eIF-2B subunit beta
• Alias Symbols: EIF2B, EIF-2Bbeta
• NCBI Gene Id: 8892
• Protein Name: Translation initiation factor eIF-2B subunit beta
• Uniprot ID: P49770
• Protein Accession #: NP_055054.1
• Molecular Weight: 39 kDa