Catalog No: OKCD02521
Size:96WELLS
Price: $700.00
SKU
OKCD02521
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Datasheets/ManualsPrintable datasheet for EIF2AK3 ELISA Kit (Mouse) (OKCD02521)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EIF2AK3 Microplate96 Wells (12 x 8 Well strips)
EIF2AK3 Lyophilized Standard2
100X Biotinylated EIF2AK3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3 (EIF2aK3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolEif2ak3
Gene Full Nameeukaryotic translation initiation factor 2 alpha kinase 3
Alias Symbolseukaryotic translation initiation factor 2-alpha kinase 3;pancreatic eIF2-alpha kinase;PE;Pek;Perk;PRKR-like endoplasmic reticulum kinase.
NCBI Gene Id13666
Protein NameEukaryotic translation initiation factor 2-alpha kinase 3
Description of TargetMetabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability (PubMed:11106749). Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:23921556). Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (PubMed:11035797). Involved in control of mitochondrial morphology and function (PubMed:23921556).
Uniprot IDQ9Z2B5
Protein Accession #NP_001300847.1
Nucleotide Accession #NM_001313918.1
Protein Size (# AA)1114
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