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Datasheets/Manuals | Printable datasheet for EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | PEK/PERK (Phospho-Thr981) antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 981. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:40000 |
Reference | Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999). Sood R., Biochem. J. 346:281-293(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004). |
Gene Symbol | EIF2AK3 |
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Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 3 |
Alias Symbols | eukaryotic translation initiation factor 2-alpha kinase 3;pancreatic EIF2-alpha kinase;PEK;PERK;PRKR-like endoplasmic reticulum kinase;truncated eukaryotic translation initiation factor 2 alpha kinase 3;WRS. |
NCBI Gene Id | 9451 |
Protein Name | Eukaryotic translation initiation factor 2-alpha kinase 3 |
Description of Target | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. |
Uniprot ID | Q9NZJ5 |
Protein Accession # | NP_004827.4 |
Molecular Weight | 125 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
This target may also be called "eukaryotic translation initiation factor 2-alpha kinase 3;pancreatic EIF2-alpha kinase;PEK;PERK;PRKR-like endoplasmic reticulum kinase;truncated eukaryotic translation initiation factor 2 alpha kinase 3;WRS." in publications.
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What is the shipping cost for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "125 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF2AK3 Antibody (Phospho-Thr981) (OAAF00471)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF2AK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF2AK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF2AK3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF2AK3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF2AK3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF2AK3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.