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Datasheets/Manuals | Printable datasheet for EIF2AK1 Antibody (OAAF03655) |
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Predicted Species Reactivity | Human|Monkey|Mouse |
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Product Format | Liquid.PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human EIF2AK1. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen. |
Specificity | EIF2AK1 Antibody detects endogenous levels of total EIF2AK1 protein. |
Application Info | WB: 1:500~1:3,000 ELISA: 1:40,000 Optimal dilutions should be determined by the end user. |
Reference | Hwang S.-Y., Mol. Cells 10:584-591(2000). Cannon G., Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases. Nagase T., DNA Res. 7:65-73(2000). |
Gene Symbol | EIF2AK1 |
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Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 1 |
Alias Symbols | eukaryotic translation initiation factor 2-alpha kinase 1;HCR;heme regulated initiation factor 2 alpha kinase;heme sensitive initiation factor 2a kinase;heme-controlled repressor;heme-regulated eukaryotic initiation factor eIF-2-alpha kinase;heme-regulated inhibitor;heme-regulated repressor;hemin-sensitive initiation factor 2-alpha kinase;hHRI;HRI;LEMSPAD. |
NCBI Gene Id | 27102 |
Protein Name | Eukaryotic translation initiation factor 2-alpha kinase 1 |
Description of Target | Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity). |
Uniprot ID | Q9BQI3 |
Protein Accession # | NP_001127807.1 |
Molecular Weight | 71 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF2AK1 Antibody (OAAF03655)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse".
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How long will it take to receive "EIF2AK1 Antibody (OAAF03655)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EIF2AK1 Antibody (OAAF03655)" provided in?
This item is provided in "Liquid.PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF2AK1 Antibody (OAAF03655)"?
This target may also be called "eukaryotic translation initiation factor 2-alpha kinase 1;HCR;heme regulated initiation factor 2 alpha kinase;heme sensitive initiation factor 2a kinase;heme-controlled repressor;heme-regulated eukaryotic initiation factor eIF-2-alpha kinase;heme-regulated inhibitor;heme-regulated repressor;hemin-sensitive initiation factor 2-alpha kinase;hHRI;HRI;LEMSPAD." in publications.
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What is the shipping cost for "EIF2AK1 Antibody (OAAF03655)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF2AK1 Antibody (OAAF03655)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF2AK1 Antibody (OAAF03655)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "71 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF2AK1 Antibody (OAAF03655)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF2AK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF2AK1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF2AK1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF2AK1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF2AK1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF2AK1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.