EGR1 ELISA Kit (Mouse) (OKCD02519)

Catalog No: OKCD02519

Size:96WELLS

Mouse EGR1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Growth Response Protein 1 (EGR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Early Growth Response Protein 1 (EGR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Growth Response Protein 1 (EGR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Early Growth Response Protein 1 (EGR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EGR1 Microplate: 96 Wells (12 x 8 Well strips)
  • EGR1 Lyophilized Standard: 2
  • 100X Biotinylated EGR1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Early Growth Response Protein 1 (EGR1). No significant cross-reactivity or interference between Early Growth Response Protein 1 (EGR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Egr1
• Gene Full Name: early growth response 1
• Alias Symbols: A530045N19Rik;early growth response protein 1;egr;Egr-;Egr-1;ETR10;ETR103;Krox;Krox-;Krox-1;Krox24;Krox-24;Krox-24 nuclear protein;nerve growth factor-induced protein A;NG;NGF;NGF1;NGF1-A;NGFIA;NGFI-A;TI;TIS8;transcription factor Zif268;Zen;Zenk;Zfp-;Zfp-6;Zif26;Zif268;zinc finger protein Krox-24.
• NCBI Gene Id: 13653
• Protein Name: Early growth response protein 1
• Description of Target: Transcriptional regulator (PubMed:8336701, PubMed:8703054, PubMed:15958557). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (PubMed:8703054, PubMed:15958557, PubMed:2028256, PubMed:8939742). Binds double-stranded target DNA, irrespective of the cytosine methylation status (By similarity). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia (PubMed:11100120, PubMed:15958557). Plays a role in the regulation of cell survival, proliferation and cell death (PubMed:15265859, PubMed:15958557). Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation (PubMed:15958557). Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy (PubMed:15265859). Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia (PubMed:11100120). Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (PubMed:8703054).
• Uniprot ID: P08046
• Protein Accession #: NP_031939.1
• Nucleotide Accession #: NM_007913.5
• Protein Size (# AA): 533