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Datasheets/Manuals | Printable datasheet for EGR-1 Antibody (OARA03138) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid (sterile filtered) |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Western blot |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1. |
Purification | The product was affinity purified from monospecific antiserum by immunoaffinity purification. |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; chimpanzee |
Concentration | 0.93 mg/ml |
Specificity | This affinity purified antibody is directed against human EGR-1 protein. |
Application Info | ELISA: 1:4,000 - 1:16,000 WB: 1:500 - 1:3,000 IHC: 2 ug/ml to 20 ug/ml |
Application Data | This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract. |
Reference | Suggs,S.V., Katzowitz,J.L., Tsai-Morris,C. and Sukhatme,V.P. (1990) cDNA sequence of the human cellular early growth response gene Egr-1. Nucleic Acids Res. 18 (14), 4283. Shimizu,N., Ohta,M., Fujiwara,C., Sagara,J., Mochizuki,N., Oda,T. and Utiyama,H. (1992) A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation. J. Biochem. 111 (2), 272-277. Wright,J.J., Gunter,K.C., Mitsuya,H., Irving,S.G., Kelly,K. and Siebenlist,U. (1990) Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells. Science 248 (4955), 588-591. |
Gene Symbol | EGR1 |
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Gene Full Name | early growth response 1 |
Alias Symbols | AT225;early growth response protein 1;EGR-1;G0S30;KROX-24;nerve growth factor-induced protein A;NGFI-A;TIS8;transcription factor ETR103;transcription factor Zif268;ZIF-268;zinc finger gene 225;zinc finger protein 225;zinc finger protein Krox-24;ZNF225. |
NCBI Gene Id | 1958 |
Protein Name | Early growth response protein 1|early growth response protein 1 [Homo sapiens] |
Description of Target | Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity). |
Uniprot ID | P18146 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001955.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EGR-1 Antibody (OARA03138)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "EGR-1 Antibody (OARA03138)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "EGR-1 Antibody (OARA03138)" provided in?
This item is provided in "Liquid (sterile filtered)".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EGR-1 Antibody (OARA03138)"?
This target may also be called "AT225;early growth response protein 1;EGR-1;G0S30;KROX-24;nerve growth factor-induced protein A;NGFI-A;TIS8;transcription factor ETR103;transcription factor Zif268;ZIF-268;zinc finger gene 225;zinc finger protein 225;zinc finger protein Krox-24;ZNF225." in publications.
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What is the shipping cost for "EGR-1 Antibody (OARA03138)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EGR-1 Antibody (OARA03138)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EGR-1 Antibody (OARA03138)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EGR-1 Antibody (OARA03138)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EGR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EGR1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EGR1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EGR1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EGR1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EGR1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.