EGFR ELISA Kit (Human) : 96 Wells (OKEH02730)

Catalog No: OKEH02730

Size:96T

Human EGFR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology EGFR ELISA Kit (Human) (OKEH02730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EGFR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for EGFR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EGFR captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.1% (n = 20)
  Mean Inter-assay CV%: < 8.2% (n = 20)
• ELISA Kit Component:
  • Anti-EGFR Microplate: 96 Wells (12 x 8 Well strips)
  • EGFR Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated EGFR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 31 pg/mL
• Specificity: Natural and recombinant Human Epidermal growth factor receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EGFR
• Gene Full Name: epidermal growth factor receptor
• Alias Symbols: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor tyrosine kinase domain;ERBB;ERBB1;erb-b2 receptor tyrosine kinase 1;ERRP;HER1;mENA;NISBD2;PIG61;proto-oncogene c-ErbB-1;receptor tyrosine-protein kinase erbB-1.
• NCBI Gene Id: 1956
• Protein Name: P00533
• Description of Target: Epidermal growth factor receptor
• Uniprot ID: https://www.uniprot.org/uniprot/P00533
• Protein Accession #: NP_005219.2
• Nucleotide Accession #: NM_005228.3
• Protein Size (# AA): 1210
• Molecular Weight: 134 kDa