Catalog No: OKCD02517
Size:96WELLS
Price: $750.00
SKU
OKCD02517
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Datasheets/ManualsPrintable datasheet for EGFL7 ELISA Kit (Mouse) (OKCD02517)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)87-99%84-94%84-91%87-98%
EDTA Plasma (n=5)80-89%98-105%84-102%84-102%
Heparin Plasma (n=5)92-101%98-105%98-105%80-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-10192
EDTA Plasma (n=5)98-105102
Heparin Plasma(n=5)90-10497
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EGFL7 Microplate96 Wells (12 x 8 Well strips)
EGFL7 Lyophilized Standard2
100X Biotinylated EGFL7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of EGF Like Domain Protein, Multiple 7 (EGFL7). No significant cross-reactivity or interference between EGF Like Domain Protein, Multiple 7 (EGFL7) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolEgfl7
Gene Full NameEGF-like domain 7
Alias SymbolsEGF-like protein 7;epidermal growth factor-like protein 7;multiple EGF-like domains protein 7;multiple epidermal growth factor-like domains protein 7;NEU1 protein;NOTCH4-like protein;vascular endothelial statin;VE-s;VE-statin;Zneu;Zneu1.
NCBI Gene Id353156
Protein NameEpidermal growth factor-like protein 7
Description of TargetRegulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
Uniprot IDQ9QXT5
Protein Accession #NP_001158036.1
Nucleotide Accession #NM_001164564.1
Protein Size (# AA)275
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