EG-VEGF ELISA Kit (Human) (OKBB00283)

Catalog No: OKBB00283

Size:96 Tests

Human PROK1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human EG-VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EG-VEGF has been precoated onto 96-well plates. Standards(NS0, A20-F105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EG-VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human EG-VEGF amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	108	228	507	98	239	512
  St. Dev.	5.94	10.26	21.8	5.59	17.69	34.82
  %CV	5.5	4.5	4.29	5.70	7.40	6.80

• ELISA Kit Component:
  • Lyophilized recombinant human EG-VEGF standard: 10 ng/tube x 2
  • Anti-human EG-VEGF Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human EG-VEGFantibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Principle: Aviva’s Human EG-VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EG-VEGF has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A20-F105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EG-VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EG-VEGF amount of sample captured in plate.
  
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.<
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <7 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine
• Reference: 1. LeCouter, J., Kowalski, J., Foster, J., Hass, P., Zhang, Z., Dillard-Telm, L., Frantz, G., Rangell, L., DeGuzman, L.,
  Keller, G.-A., Peale, F., Gurney, P., Hillan, K. J., Ferrara, N. Identification of an angiogenic mitogen selective for endocrine gland endothelium. Nature 412: 877-884, 2001. [PubMed: 11528470].
  2. Li, M., Bullock, C. M., Knauer, D. J., Ehlert, F. J., Zhou, Q. Y. Identification of two prokineticin cDNAs: recombinant
  proteins potently contract gastrointestinal smooth muscle. Molec. Pharm. 59: 692-698, 2001. [PubMed: 11259612]
• Specificity: Natural and recombinant human EG-VEGF
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A20-F105
• Description: For quantitative detection of human EG-VEGF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: PROK1
• Gene Full Name: prokineticin 1
• Alias Symbols: EG-VEGF, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, PROK1, PROK1_HUMAN, Prokineticin-1
• NCBI Gene Id: 84432
• Protein Name: Prokineticin-1
• Description of Target: EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands.
• Uniprot ID: P58294
• Protein Accession #: NP_115790.1
• Nucleotide Accession #: NM_032414.2