Catalog No: OKCD00700 (Formerly GWB-KBBOU7)
Size:96WELLS
Price: $675.00
SKU
OKCD00700
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Datasheets/ManualsPrintable datasheet for EFEMP1 ELISA Kit (Human) (OKCD00700)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-93%93-104%80-91%92-105%
EDTA plasma(n=5)86-103%83-94%95-105%90-97%
heparin plasma(n=5)84-103%92-101%96-104%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-10197
EDTA plasma(n=5)92-10197
heparin plasma(n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EFEMP1 Microplate96 Wells (12 x 8 Well Strips)
EFEMP1 Lyophilized Standard2 x 100 ng
100X Biotinylated EFEMP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, urine and other biological fluids
Sensitivity< 0.64 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibulin 3 (FBLN3). No significant cross-reactivity or interference between Fibulin 3 (FBLN3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEFEMP1
Gene Full NameEGF containing fibulin extracellular matrix protein 1
Alias SymbolsDHRD;DRAD;EGF containing fibulin like extracellular matrix protein 1;EGF-containing fibulin-like extracellular matrix protein 1;extracellular protein S1-5;FBLN3;FBNL;FIBL-3;Fibrillin-like protein;fibulin-3;MLVT;MTLV;S1-5.
NCBI Gene Id2202
Protein NameEGF-containing fibulin-like extracellular matrix protein 1
Description of TargetBinds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Uniprot IDQ12805
Protein Accession #NP_001034437
Nucleotide Accession #NM_001039348.2
Protein Size (# AA)493
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