EEF1G ELISA Kit (Human) (OKCD00773)

Catalog No: OKCD00773 (Formerly GWB-KBBKV9)

Size:96WELLS

Human EEF1G ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.25-2000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EEF1G Microplate: 96 Wells (12 x 8 Well Strips)
  • EEF1G Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated EEF1G Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 12.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EEF1G
• Gene Full Name: eukaryotic translation elongation factor 1 gamma
• Alias Symbols: eEF-1B gamma;EF1G;EF-1-gamma;elongation factor 1-gamma;GIG35;pancreatic tumor-related protein;PRO1608;translation elongation factor eEF-1 gamma chain.
• NCBI Gene Id: 1937
• Protein Name: Elongation factor 1-gamma
• Description of Target: Probably plays a role in anchoring the complex to other cellular components.
• Uniprot ID: P26641
• Protein Accession #: NP_001395
• Nucleotide Accession #: NM_001404.4
• Protein Size (# AA): 437