Catalog No: OKCD02399
Size:96WELLS
Price: $725.00
SKU
OKCD02399
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Datasheets/ManualsPrintable datasheet for EDN2 ELISA Kit (Rat) (OKCD02399)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%78-102%89-97%94-103%
EDTA Plasma (n=5)80-98%87-97%97-104%83-95%
Heparin Plasma (n=5)93-101%97-105%79-101%88-98%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 2 (EDN2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 2 (EDN2) and unlabeled Endothelin 2 (EDN2) (Standards or samples) with the pre-coated antibody specific to Endothelin 2 (EDN2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 2 (EDN2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 2 (EDN2) in the sample.
ELISA Kit Range6.17-500pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)85-9988
EDTA Plasma (n=5)90-10197
Heparin Plasma (n=5)81-9987
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 2 (EDN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 2 (EDN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Edn2 Microplate96 Wells (12 x 8 Well strips)
Edn2 Lyophilized Standard 2 x 500 pg
100X Edn2 Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 2.67 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Endothelin 2 (EDN2). No significant cross-reactivity or interference between Endothelin 2 (EDN2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Inhibition ELISA
Gene SymbolEDN2
Gene Full NameEndothelin 2
Alias SymbolsPreproendothelin-2;Vasoactive intestinal contractor.
NCBI Gene Id24324
Protein NameEndothelin-2
Description of TargetVasoconstrictor.
Uniprot IDP23943
Protein Accession #NP_036681.2
Nucleotide Accession #NM_012549.3
Molecular Weight176 kDa
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