DYX1C1 Antibody (OAEB01476)

Catalog No: OAEB01476 (Formerly GWB-379439)

Size:100UG

OAEB01476 staining (0.1ug/ml) of COS1 cell lysates: untransfected (left lane) and transfected with full length recombinant Human DYX1C1 (right lane). Data kindly provided by Wang and LoTurco, University of Connecticut, USA.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: DNAAF4
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 90%; Dog: 76%; Mouse: 84%; Pig: 92%; Rat: 84%
• Peptide Sequence: C-KIRNVIQGTELKS
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1ug/ml antibody staining. In addition,
  a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.
• Reference: * Wang Y, Paramasivam M, Thomas A, Bai J, Kaminen-Ahola N, Kere J, Voskuil J, Rosen GD, Galaburda AM, Loturco JJ.; DYX1C1 functions in neuronal migration in developing neocortex.; Neuroscience. 2006 Dec 1;143(2):515-522. Epub 2006 Sep 20.; Pubmed 16989952
  * Taipale M, Kaminen N, Nopola-Hemmi J, Haltia T, Myllyluoma B, Lyytinen H, Muller K, Kaaranen M, Lindsberg PJ, Hannula-Jouppi K, Kere J.; A candidate gene for developmental dyslexia encodes a nuclear tetratricopeptide repeat domain protein dynamically regulated in brain.; Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11553-8. Epub 2003 Sep 03.; Pubmed 12954984

Target Info

• Gene Symbol: Dnaaf4|DNAAF4
• Gene Full Name: dynein axonemal assembly factor 4
• Alias Symbols: 1700010I24Rik;b2b811.1Clo;b2b811C;b2b811Clo;CILD25;dynein assembly factor 4, axonemal;dynein axonemal assembly factor 4;dyslexia susceptibility 1 candidate 1;dyslexia susceptibility 1 candidate 1 homolog;dyslexia susceptibility 1 candidate gene 1 protein;dyslexia susceptibility 1 candidate gene 1 protein homolog;DYX1;DYX1C1;DYXC1;EKN;EKN1;RD.
• NCBI Gene Id: 161582|67685
• Protein Name: Dyslexia susceptibility 1 candidate gene 1 protein
• Uniprot ID: Q8WXU2
• Protein Accession #: NP_570722.2
• Molecular Weight: 48.5