Catalog No: OKCD01050 (Formerly GWB-KBBIE5)
Size:96WELLS
Price: $675.00
SKU
OKCD01050
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for DYRK1A ELISA Kit (Human) (OKCD01050) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.117 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). No significant cross-reactivity or interference between Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | DYRK1A |
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Gene Full Name | dual specificity tyrosine phosphorylation regulated kinase 1A |
Alias Symbols | dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A;dual specificity tyrosine-phosphorylation-regulated kinase 1A;dual specificity YAK1-related kinase;DYRK;DYRK1;HP86;MNB;mnb protein kinase homolog hp86;MNB/DYRK protein kinase;MNBH;MRD7;protein kinase minibrain homolog;serine/threonine kinase MNB;serine/threonine-specific protein kinase. |
NCBI Gene Id | 1859 |
Protein Name | Dual specificity tyrosine-phosphorylation-regulated kinase 1A |
Description of Target | Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity). |
Uniprot ID | Q13627 |
Protein Accession # | NP_001387 |
Nucleotide Accession # | NM_001396.3 |
Protein Size (# AA) | 763 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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