DUSP6 ELISA Kit (Human) (OKCD00989)

Catalog No: OKCD00989 (Formerly GWB-KBBQW9)

Size:96WELLS

Human DUSP6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 6 (DUSP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 6 (DUSP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 6 (DUSP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Specificity Phosphatase 6 (DUSP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 6 (DUSP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 6 (DUSP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DUSP6 Microplate: 96 Wells (12 x 8 Well Strips)
  • DUSP6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DUSP6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 6 (DUSP6). No significant cross-reactivity or interference between Dual Specificity Phosphatase 6 (DUSP6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DUSP6
• Gene Full Name: dual specificity phosphatase 6
• Alias Symbols: dual specificity protein phosphatase 6;dual specificity protein phosphatase PYST1;HH19;MAP kinase phosphatase 3;mitogen-activated protein kinase phosphatase 3;MKP3;PYST1;serine/threonine specific protein phosphatase.
• NCBI Gene Id: 1848
• Protein Name: Dual specificity protein phosphatase 6
• Description of Target: Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:9858808). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity).
• Uniprot ID: Q16828
• Protein Accession #: NP_001937
• Nucleotide Accession #: NM_001946.3
• Protein Size (# AA): 381