Catalog No: OKCD00485 (Formerly GWB-KBBOI8)
Size:96WELLS
Price: $700.00
SKU
OKCD00485
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Datasheets/ManualsPrintable datasheet for DUSP5 ELISA Kit (Mouse) (OKCD00485)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 5 (DUSP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Specificity Phosphatase 5 (DUSP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Dusp5 Microplate96 Wells (12 x 8 Well Strips)
Dusp5 Lyophilized Standard2 x 10 ng
100X Biotinylated Dusp5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 5 (DUSP5). No significant cross-reactivity or interference between Dual Specificity Phosphatase 5 (DUSP5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDusp5
Gene Full Namedual specificity phosphatase 5
Alias Symbolsdual specificity protein phosphatase 5;Gm337.
NCBI Gene Id240672
Protein NameDual specificity protein phosphatase
Description of TargetDual specificity protein phosphatase
Uniprot IDQ1HL35
Protein Accession #NP_001078859
Nucleotide Accession #NM_001085390.1
Protein Size (# AA)384
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