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Catalog No: OKCD00463 (Formerly GWB-KBBZW8)
Size:96WELLS
Price: $675.00
SKU
OKCD00463
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Datasheets/ManualsPrintable datasheet for DTNB ELISA Kit (Human) (OKCD00463)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrobrevin Beta (DTNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrobrevin Beta (DTNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrobrevin Beta (DTNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dystrobrevin Beta (DTNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrobrevin Beta (DTNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrobrevin Beta (DTNb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DTNB Microplate96 Wells (12 x 8 Well Strips)
DTNB Lyophilized Standard2 x 10 ng
100X Biotinylated DTNB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.053 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dystrobrevin Beta (DTNb). No significant cross-reactivity or interference between Dystrobrevin Beta (DTNb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDTNB
Gene Full Namedystrobrevin beta
Alias Symbolsbeta-dystrobrevin;DTN-B;dystrobrevin beta.
NCBI Gene Id1838
Protein NameDystrobrevin beta
Description of TargetDystrobrevin beta
Uniprot IDO60941
Protein Accession #NP_001243232
Nucleotide Accession #NM_001256303.1
Protein Size (# AA)627
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