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Catalog No: OKEH03692
Size:96T
Price: $625.00
SKU
OKEH03692
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DST ELISA Kit (Mouse) (OKEH03692) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dst has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dst is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dst captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Dst Microplate96 Wells (12 x 8 Well strips)
Dst Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Dst Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Dystonin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDst
Gene Full Namedystonin
Alias Symbols2310001O04Rik;a;A830042E19Rik;ah;athet;athetoid;AW554249;Bp;BP230;BPA;Bpag;Bpag1;BPAG1-n;Bullous pemphigoid antigen 1;dt;dystonia musculorum protein;dystonin;hemidesmosomal plaque protein;Macf;Macf2;microtubule actin cross-linking factor 2;mKIAA0728;nmf20;nmf203;nmf33;nmf339.
NCBI Gene Id13518
Protein NameQ91ZU6
Description of TargetDystonin
Uniprot IDhttps://www.uniprot.org/uniprot/Q91ZU6
Protein Accession #NP_034211.2
Nucleotide Accession #NM_010081.2
Protein Size (# AA)7393
Molecular Weight834 kDa
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