Catalog No: OKCA00019
Size:96 Wells
Price: $550.00
SKU
OKCA00019
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Human | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology dsDNA Antibody (IgG) ELISA Kit (Human) (OKCA00019) is based on a qualitative reverse phase enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with dsDNA antigen and blocked. Samples or controls are added to the plate wells, incubated and washed. A horseradish peroxidase (HRP) conjugated anti-human IgG detector antibody is then added, incubated and washed.dsDNA Antibody in samples or controls will bind to the antigen immobilized in the well and are subsequently bound by the HRP conjugated anti-human IgG antibody. Washing removes unbound sample and detector antibody. An enzymatic reaction is then produced through the addition of reporter substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is proportional to the amount of dsDNA Antibody contained in the sample or standard. | ||||||||||||||||||||
ELISA Kit Range | Request Information | ||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay: CV% < 15% (n = 20). Inter-assay: CV% < 15% (n = 20) | ||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | serum | ||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of human anti-double stranded DNA (dsDNA) antibody (IgG). No significant cross-reactivity or interference between human anti-cardiolipin antibody(IgM) and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human anti-cardiolipin antibody(IgM) and all the analogues, therefore, cross reaction may still exist. | ||||||||||||||||||||
Assay Info | Assay Methodology: Qualitative Reverse Capture Immunoassay |
Gene Symbol | dsDNA IgG |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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