Catalog No: OKCA00019
Size:96 Wells
Price: $550.00
SKU
OKCA00019
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dsDNA Antibody (IgG) ELISA Kit (Human) : 96 Wells (OKCA00019)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKCA00019
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology dsDNA Antibody (IgG) ELISA Kit (Human) (OKCA00019) is based on a qualitative reverse phase enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with dsDNA antigen and blocked. Samples or controls are added to the plate wells, incubated and washed. A horseradish peroxidase (HRP) conjugated anti-human IgG detector antibody is then added, incubated and washed.dsDNA Antibody in samples or controls will bind to the antigen immobilized in the well and are subsequently bound by the HRP conjugated anti-human IgG antibody. Washing removes unbound sample and detector antibody. An enzymatic reaction is then produced through the addition of reporter substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is proportional to the amount of dsDNA Antibody contained in the sample or standard.
ELISA Kit RangeRequest Information
ELISA Kit ReproducibilityIntra-assay: CV% < 15% (n = 20). Inter-assay: CV% < 15% (n = 20)
ELISA Kit Component
ComponentAmount
dsDNA Microplate96 Well (12 x 8 Well Strips)
Negative Control1 x 800 ul
Positive Control1 x 800 ul
Sample Diluent1 x 20 mL
Anti-Human IgG Antibody HRP-Conjugate1 x 10 mL
25X Wash Buffer1 x 20 mL
Substrate A1 x 5 mL
Substrate B1 x 5 mL
Stop Solution1 x 5 mL
Reconstitution and StorageStore as indicated in product manual.
Sample Typeserum
SpecificityThis assay has high sensitivity and excellent specificity for detection of human anti-double stranded DNA (dsDNA) antibody (IgG). No significant cross-reactivity or interference between human anti-cardiolipin antibody(IgM) and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human anti-cardiolipin antibody(IgM) and all the analogues, therefore, cross reaction may still exist.
Assay InfoAssay Methodology: Qualitative Reverse Capture Immunoassay
Gene SymboldsDNA IgG
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