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Catalog No: OKDD00230 (Formerly GWB-KBBXA5)
Size:96 Wells
Price: $750.00
SKU
OKDD00230
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Datasheets/ManualsPrintable datasheet for DLAT ELISA Kit (Mouse) (OKDD00230)
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology DLAT ELISA Kit (Mouse) (OKDD00230) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for DLAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DLAT is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DLAT captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DLAT were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DLAT were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-DLAT Microplate96 Wells (12 x 8 Well Strips)
DLAT Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DLAT Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.048 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of DLAT. No significant cross-reactivity or interference between DLAT and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolDlat
Gene Full Namedihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Alias Symbols6332404G05Rik;dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex;dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial;DLTA;PDC-E;PDCE2;PDC-E2;pyruvate dehydrogenase complex component E2.
NCBI Gene Id235339
Protein NameDihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Description of TargetThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Uniprot IDQ8BMF4
Protein Accession #NP_663589.3
Nucleotide Accession #NM_145614.4
Protein Size (# AA)642
Molecular Weight68 kDa
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