DKK3 ELISA Kit (Mouse) (OKCD02498)

Catalog No: OKCD02498

Size:96WELLS

Mouse DKK3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	81-103%	90-104%	93-105%
  EDTA Plasma (n=5)	98-105%	93-101%	98-105%	79-99%
  Heparin Plasma (n=5)	90-98%	78-89%	93-101%	88-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 3 (DKK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 3 (DKK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 3 (DKK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dickkopf Related Protein 3 (DKK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-102	96
  EDTA Plasma (n=5)	78-103	86
  Heparin Plasma(n=5)	80-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DKK3 Microplate: 96 Wells (12 x 8 Well strips)
  • DKK3 Lyophilized Standard: 2
  • 100X Biotinylated DKK3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.
• Publications:

  A combined bioinformatics, experimental and clinical approach to identify novel cardiac-specific heart failure biomarkers: is Dickkopf-3 (DKK3) a possible candidate?. Eur J Heart Fail. 22, 2065-2074 (2020). 32809235

  Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for DKK3 ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: Dkk3
• Gene Full Name: dickkopf WNT signaling pathway inhibitor 3
• Alias Symbols: AW061014;C87148;dickkopf homolog 3;dickkopf-3;dickkopf-related protein 3;dkk-3;mDkk-3.
• NCBI Gene Id: 50781
• Protein Name: Dickkopf-related protein 3
• Description of Target: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
• Uniprot ID: Q9QUN9
• Protein Accession #: NP_056629.1
• Nucleotide Accession #: NM_015814.2
• Protein Size (# AA): 349