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Catalog No: OKBB00461
Size:96 Wells
Price: $583.00
SKU
OKBB00461
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for DKK1 has been precoated onto 96-well plates. Standards(CHO, S30-H272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse DKK1 amount of sample captured in plate.
ELISA Kit Range46.9 pg/ml - 3,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)22884514742598971532
SD12.5456.691.3917.166.4105.7
CV%5.56.76.26.67.46.9
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse DKK1 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse DKK1 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse DKK1 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<15 pg/ml
SpecificityNatural and recombinant mouse DKK1
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: CHO; Immunogen sequence: S30-H272
DescriptionFor quantitative detection of mouse DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolDKK1
Gene Full Namedickkopf WNT signaling pathway inhibitor 1
Alias SymbolsDickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK
NCBI Gene Id13380
Protein NameDickkopf-related protein 1
Description of TargetDickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Uniprot IDO54908
Protein Accession #NP_034181.2
Nucleotide Accession #NM_010051.3
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