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Catalog No: OKEH02724
Size:96T
Price: $525.00
SKU
OKEH02724
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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DIO2 ELISA Kit (Human) : 96 Wells (OKEH02724)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DIO2 ELISA Kit (Human) (OKEH02724) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human DIO2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DIO2 and incubated. The DIO2 found in the sample or standards competes with the biotinylated DIO2 for limited binding sites on the immobilized anti-Human DIO2 antibody. Excess unbound biotinylated DIO2 and sample or standard DIO2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DIO2 captured in the well and inversely proportional to the amount of DIO2 which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-DIO2 Microplate96 Wells (12 x 8 Well strips)
DIO2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X DIO2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Type II iodothyronine deiodinase
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolDIO2
Gene Full Nameiodothyronine deiodinase 2
Alias Symbols5DII;D2;deiodinase, iodothyronine type II;deiodinase-2;deiodonase-2;DIOII;selenoprotein Y;SELENOY;SelY;thyroxine deiodinase, type II;TXDI2;type 2 DI;type 2 iodothyronine deiodinase;type II iodothyronine deiodinase;type-II 5'deiodinase;type-II 5'-deiodinase.
NCBI Gene Id1734
Protein NameQ92813
Description of TargetType II iodothyronine deiodinase
Uniprot IDhttps://www.uniprot.org/uniprot/Q92813
Protein Accession #NP_000784.2
Nucleotide Accession #NM_000793.5
Protein Size (# AA)273
Molecular Weight31 kDa
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