Catalog No: OKEH06590
Size:96T
Price: $625.00
SKU
OKEH06590
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Gabra2 ELISA Kit (Bovine) (OKEH06590) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gabra2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gabra2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gabra2 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/ml | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 19 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Bovine Gamma-aminobutyric acid receptor subunit alpha-2 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | GABRA2 |
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Gene Full Name | gamma-aminobutyric acid type A receptor alpha2 subunit, gamma-aminobutyric acid type A receptor subunit alpha2 |
Alias Symbols | GABA(A) receptor subunit alpha-2;gamma-aminobutyric acid (GABA) A receptor, alpha 2;gamma-aminobutyric acid receptor subunit alpha-2;gamma-aminobutyric acid type A receptor alpha2 subunit. |
NCBI Gene Id | 104972746, 282236 |
Protein Name | P10063 |
Description of Target | Gamma-aminobutyric acid receptor subunit alpha-2 |
Uniprot ID | https://www.uniprot.org/uniprot/P10063 |
Protein Accession # | NP_776966 |
Nucleotide Accession # | NM_174541 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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