Catalog No: OKEH02470
Size:96T
Price: $625.00
SKU
OKEH02470
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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DID ELISA Kit (Rat) : 96 Wells (OKEH02470)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DID ELISA Kit (Rat) (OKEH02470) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat DID antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DID and incubated. The DID found in the sample or standards competes with the biotinylated DID for limited binding sites on the immobilized anti-Rat DID antibody. Excess unbound biotinylated DID and sample or standard DID is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DID captured in the well and inversely proportional to the amount of DID which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-DID Microplate96 Wells (12 x 8 Well strips)
DID Lyophilized Standard2
Sample Diluent1 x 20 mL
100X BAD-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.47 ng/mL
SpecificityNatural and recombinant Rat Dihydrolipoyl dehydrogenase, mitochondrial
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolDld
Gene Full Namedihydrolipoamide dehydrogenase
Alias SymbolsDihydrolipoamide dehydrogenase;dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex);dihydrolipoyl dehydrogenase, mitochondrial.
NCBI Gene Id298942
Protein NameQ6P6R2
Description of TargetDihydrolipoyl dehydrogenase, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q6P6R2
Protein Accession #NP_955417.1
Nucleotide Accession #NM_199385.2
Protein Size (# AA)509
Molecular Weight54 kDa
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