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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-DFFA (OAAN00178) |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | WB, IHC, IF |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Recombinant protein of human DFFA |
Purification | Affinity purified against immunogen |
Preservative | 0.02% Sodium Azide |
Application Info | WB: 1:500 - 1:2,000. IHC: 1:50 - 1:200. IF: 1:50 - 1:100 |
Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Gene Symbol | DFFA |
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Gene Full Name | DNA fragmentation factor subunit alpha |
Alias Symbols | DFF1, ICAD, DFF-45 |
NCBI Gene Id | 1676 |
Protein Name | DNA fragmentation factor subunit alpha |
Description of Target | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Uniprot ID | O00273 |
Protein Accession # | NP_004392.1 |
Nucleotide Accession # | NM_004401.2 |
Molecular Weight | 37 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "DFFA Antibody (OAAN00178)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "DFFA Antibody (OAAN00178)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "DFFA Antibody (OAAN00178)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "DFFA Antibody (OAAN00178)"?
This target may also be called "DFF1, ICAD, DFF-45" in publications.
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What is the shipping cost for "DFFA Antibody (OAAN00178)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "DFFA Antibody (OAAN00178)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "DFFA Antibody (OAAN00178)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "37 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "DFFA Antibody (OAAN00178)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "DFFA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "DFFA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "DFFA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "DFFA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "DFFA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "DFFA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.