DES Antibody (OAEB01539)

Catalog No: OAEB01539 (Formerly GWB-EV0472)

Size:100UG

OAEB01539 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB01539 (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, WB, IHC
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: DES
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-RDGEVVSEATQQQHE
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:2000
  WB: Approx 55-58kDa band in Mouse Skeletal Muscle lysate (calculated MW of 53.5kDa according to Mouse NP_034173.1). Recommended concentration: 0.3-1 ug/ml. Primary incubation 1 hour at room temperature.
  IHC: Paraffin embedded Human Heart. Recommended concentration: 8 ug/ml.
• Reference: * Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL.; Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis.; J Biol Chem. 2003 Feb 28;278(9):6848-53.; Pubmed 12477713

Target Info

• Gene Symbol: Des|DES
• Gene Full Name: desmin
• Alias Symbols: cardiomyopathy, dilated 1F (autosomal dominant);CDCD3;CSM1;CSM2;desmin;epididymis secretory sperm binding protein;intermediate filament protein;LGMD1D;LGMD1E;LGMD2R.
• NCBI Gene Id: 13346|1674|64362
• Protein Name: Desmin
• Uniprot ID: P17661
• Protein Accession #: NP_001918.3
• Molecular Weight: 53.5