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Catalog No: OKEH06511
Size:96T
Price: $525.00
SKU
OKEH06511
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Dek ELISA Kit (Mouse) (OKEH06511) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dek has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dek is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dek captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93.7%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 6.3% (n = 20)
Mean Inter-Assay Precision - <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.18 ng/mL
SpecificityNatural and recombinant Mouse Protein DEK
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolDek
Gene Full NameDEK proto-oncogene (DNA binding)
Alias Symbols1810019E15Rik;D13H6S231;D13H6S231E;DEK oncogene (DNA binding);protein DEK.
NCBI Gene Id110052
Protein NameQ7TNV0
Description of TargetProtein DEK
Uniprot IDhttps://www.uniprot.org/uniprot/Q7TNV0
Protein Accession #NP_722489
Nucleotide Accession #NM_153794
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