DEDAF Antibody (OAPB00091)

Catalog No: OAPB00091 (Formerly GWB-C31B5F)

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 μg /ml.

Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/mL.

Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 ug/ml.

Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: A549 Cell Lysate (OCPB00179), HepG2 Cell Lysate (OCPB00171), Mouse Liver Tissue Lysate (OCPB00083)
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF. The immunogen is located within the last 50 amino acids of DEDAF.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-DEDAF antibody is Catalog # OEPB02217
• Application Info: BAFF antibody can be used for detection of BAFF by Western blot a 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL. For immunofluorescence start at 20 ug/mL.
• Reference: Zheng L, Schickling O, Peter ME, Lenardo MJ. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 2001;276(34):31945-52. ;Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC, Vidal M. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18(12):3404-18. (RD0201) ;

Target Info

• Gene Symbol: RYBP
• Gene Full Name: RING1 and YY1 binding protein
• Alias Symbols: AAP1;APAP-1;apoptin-associating protein 1;death effector domain-associated factor;DEDAF;DED-associated factor;RING1 and YY1-binding protein;ring1 interactor RYBP;YEAF1;YY1 and E4TF1 associated factor 1;YY1 and E4TF1-associated factor 1.
• NCBI Gene Id: 23429
• Protein Name: Homo sapiens death effector domain-associated factor mRNA, complete cds|RING1 and YY1-binding protein
• Description of Target: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity).
• Uniprot ID: Q8N488
• Protein Accession #: AF179286
• Nucleotide Accession #: https://www.ncbi.nlm.nih.gov/nuccore/AF179286
• Molecular Weight: 32 kDa