Decorin ELISA Kit (Human) (OKBB00320)

Catalog No: OKBB00320

Size:96 Tests

Human DCN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards(sf21, G17-K359) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Decorin amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	128	645	1274	139	677	1333
  St. Dev.	5.76	41.28	72.62	8.48	56.9	89.31
  %CV	4.5	6.4	5.70	6.10	8.40	6.69

• ELISA Kit Component:
  • Lyophilized recombinant human Decorin standard: 10 ng/tube x 2
  • Anti-human Decorin Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human Decorin antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 31.2pg/ml-2000pg/ml
  Principle: Aviva’s human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards (NSO, D31-K359) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-PeroxidasComplex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Decorin amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using.
  Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Reference: 1."Entrez Gene: DCN decorin".
  2.Danielson, K. G., Fazzio, A., Cohen, I., Cannizzaro, L. A., Eichstetter, I., Iozzo, R. V. The human decorin
  gene:intron-exon organization, discovery of two alternatively spliced exons in the 5-prime untranslated region, and
  mapping of the gene to chromosome 12q23. Genomics 15: 146-160, 1993.
  3.Moscatello, D. K., Santra, M., Mann, D. M., McQuillan, D. J., Wong, A. J., Iozzo, R. V. Decorin suppresses tumor cell
  growth by activating the epidermal growth factor receptor. J. Clin. Invest. 101: 406-412, 1998.
• Specificity: Natural and recombinant human Decorin
• Immunogen: Expression system for standard: sf21; Immunogen sequence: G17-K359
• Description: For quantitative detection of human Decorin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: DCN
• Gene Full Name: decorin
• Alias Symbols: Bone proteoglycan II, CSCD, DCN, DCN protein, Decorin, Decorin proteoglycan, Dermatan sulphate proteoglycans II, DKFZp686J19238, DSPG 2, DSPG2, PG 40, PG II, PG S2, PG-S2, PG40, PGII, PGS 2, PGS2, PGS2_HUMAN, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B
• NCBI Gene Id: 1634
• Protein Name: Decorin
• Description of Target: Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size. It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
• Uniprot ID: P07585
• Protein Accession #: NP_001911.1
• Nucleotide Accession #: NM_001920.4