DDR1 ELISA Kit (Human) (OKEH02503)

Catalog No: OKEH02503

Size:96T

Human DDR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DDR1 ELISA Kit (Human) (OKEH02503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDR1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.8% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • DDR1 Microplate: 96 Wells (12 x 8 Well strips)
  • DDR1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated DDR1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.056 ng/mL
• Specificity: Natural and recombinant Human Epithelial discoidin domain-containing receptor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DDR1
• Gene Full Name: discoidin domain receptor tyrosine kinase 1
• Alias Symbols: CAK;CD167;CD167 antigen-like family member A;cell adhesion kinase;DDR;Discoidin receptor tyrosine kinase;EDDR1;epithelial discoidin domain-containing receptor 1;HGK2;mammary carcinoma kinase 10;MCK10;NEP;neuroepithelial tyrosine kinase;neurotrophic tyrosine kinase, receptor, type 4;NTRK4;Protein-tyrosine kinase 3A;protein-tyrosine kinase RTK-6;PTK3;PTK3A;PTK3A protein tyrosine kinase 3A;RTK6;TRK E;TRKE;tyrosine kinase DDR;tyrosine-protein kinase CAK.
• NCBI Gene Id: 780
• Protein Name: Q08345
• Description of Target: Epithelial discoidin domain-containing receptor 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q08345
• Protein Accession #: NP_001189450.1
• Nucleotide Accession #: NM_001202521.1
• Protein Size (# AA): 913
• Molecular Weight: 101 kDa